Cell binding fragments from a sponge proteoglycan-like aggregation factor.
نویسندگان
چکیده
منابع مشابه
Identification and further characterization of the specific cell binding fragment from sponge aggregation factor
Monoclonal antibodies (McAbs) were raised against the aggregation factor (AF) from the marine sponge Geodia cydonium. Two clones were identified that secrete McAbs against the cell binding protein of the AF complex. Fab fragments of McAbs: 5D2-D11 completely abolished the activity of the AF to form secondary aggregates from single cells. The McAbs were determined to react with the AF in vitro; ...
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Species-specific reaggregation of cells from the marine sponge Microciona prolifera is mediated by a proteoglycan-like aggregation factor (MAF) of Mr = 2 X 10(7) which has two functional domains, a cell binding domain and an aggregation factor interaction domain. After extensive trypsin digestion, over 60% of the MAF mass was converted into a glycopeptide fragment of Mr = 10,000 (T-10) which is...
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Although discriminating self from nonself is a cardinal animal trait, metazoan allorecognition genes do not appear to be homologous. Here, we characterize the Aggregation Factor (AF) gene family, which encodes putative allorecognition factors in the demosponge Amphimedon queenslandica, and trace its evolution across 24 sponge (Porifera) species. The AF locus in Amphimedon is comprised of a clus...
متن کاملMolecular conformations in proteoglycan aggregation.
that murein will eventually be discovered to require an apoprotein as the initiator, as is turning out to be the case with a number of, what were for long thought to be, ‘pure’ polysaccharides. Examples are the proteoglycans of connective tissue, and storage polysaccharides such as glycogen. No less exciting have been the developments in understanding the stereochemistry of the proteoglycans, t...
متن کاملCloning and expression of the putative aggregation factor from the marine sponge Geodia cydonium.
Sponges (phylum Porifera) have extensively been used as a model system to study cell-cell interaction on molecular level. Recently, we identified and cloned the putative aggregation receptor (AR) of the sponge Geodia cydonium, which interacts in a heterophilic way with the aggregation factor (AF) complex. In the present study, antibodies against this complex have been raised that abolish the ad...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34519-8